Protein Engineering

Protein engineering is the production of unnatural polypeptides, often through modification of amino acid sequences that are found normally in nature. Synthetic protein structures and functions can be designed entirely on a computer or produced through directed evolution in the laboratory. There are two major methods for protein engineering, rational design and irrational design, also known as directed evolution. In rational design, knowledge of the structure and function of the protein is taken into consideration and a coherent gene mutation is calculated. Mostly, this is done by making rationally designed changes in the gene of the protein cloned in expression vector of heterologous expression. The production of protein molecules is transformed by site directed or site specific mutagenesis of their genes. However, in some cases protein structure is not available and directed evolution method is required. In this method, random changes (mutation) are done in the protein and the mutant protein with the most desired characteristics is selected for further evaluation or studies.

  • Rational design
  • Multiple Sequence Alignment
  • Coevolutionary Analysis
  • Structural Prediction
  • Directed evolution

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